Mouse Human secretory component (free and bound), conjugated with Biotin Antibody

Biotin-conjugated purified monoclonal mouse antibody to human secretory component, free or bound. To identify the presence of free or bound secretory component in human milk, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining and immunoblotting. As a second step an avidin or streptavidin conjugate of the user's choice has to be used. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:20 upwards; in ELISA from 1:25 upwards; in Western blotting from 1:50 upwards. These data should be interpreted as general recommendations only.