5-TCO-PEG4-dUTP
5-TCO-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting TCO-functionalized DNA/cDNA can subsequently be labeled via Cu (I) -free Click Chemistry (TCO-Tetrazine-Ligation) that offers the choice: to introduce a Biotin group (via Tetrazines of Biotin) for subse-quent purification tasks; to introduce fluorescent group (via Tetrazines of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Tetrazine-functionalized biomolecules e.g. proteins.
Product Specifications
Product Name Alternative
5-trans-Cyclooctene-PEG4-dUTP
Concentration
1.0 mM-1.1 mM
Purity
95% (HPLC)
Form
Solution in water; Color: colorless to slightly yellow; pH: 7.5 0.5
Smiles
C (/C=C/c1cn (c (=O) [nH]c1=O) [C@H]1C[C@@H] ([C@H] (O1) COP (=O) (O) OP (=O) (O) OP (=O) (O) O) O) NC (=O) CCOCCOCCOCCOCCNC (=O) OC1CCCC=CCC1
Molecular Formula
C32H53N4O21P3
Molecular Weight
Theoretical MW: 922.70 g/mol (free acid) ; Detected MW: 922.24 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Incorporation into DNA/cDNA by PCR with Taqpolymerasein-house data. Spectroscopic Propertie: max 240 nm, 10.7 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .
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