5-DBCO-PEG4-dUTP
5-DBCO-PEG 4-dUTP is recommended for two-step labeling of DNA/cDNA e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The result-ing DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu (I) -free click chemistry that offers the choice: to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks; to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins.
Product Specifications
Product Name Alternative
5-Dibenzylcyclooctyl-PEG4-uridine-5'-triphosphate, Triethylammonium salt
Concentration
10 mM-11 mM
Purity
95% (HPLC)
Form
Solution in 100 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 0.5
Smiles
Molecular Formula
C42H54N5O21P3
Molecular Weight
Theoretical MW: 1057.83 g/mol (free acid) ; Detected MW: 1057.25 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Incorporation into DNA/cDNA by PCR with Taqpolymerasein-house data. Spectroscopic Propertie: max 289 nm, 12.5 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .
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