5-Ethynyl-2'-deoxyuridine (5-EdU)
5-EdU (5-Ethynyl-2'-deoxyuridine) can be used as a replacement for BrdU (5-Bromo-2'-deoxyuridine) to measure de novo DNA synthesis during the S-phase of the cell cycle. 5-EdU is cell permeable and incorporates into replicating DNA instead of its natural analog thymi-dine. The resulting ethynyl-functionalized DNA can subsequently be detected via Cu (I) -catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging.
Product Specifications
CAS Number
61135-33-9
Product Name Alternative
5-Ethynyl-2'-deoxyuridine
Purity
98% (HPLC)
Form
Solid; Color: white to off-white
Solubility
DMSO, up to 200 mM (at room temperature) in 1x PBS or water by heating the obtained suspension for 1 minute by 70 C
Smiles
N1 (cc (c (=O) [nH]c1=O) C#C) [C@H]1C[C@@H] ([C@H] (O1) CO) O
Molecular Formula
C11H12N2O5
Molecular Weight
Theoretical MW: 252.23 g/mol; Detected MW: 252.07 g/mol
Storage Conditions
Store at -20 °C. store dry and under inert gas. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
DNA synthesis monitoring. Spectroscopic Propertie: max 288 nm, 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .
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