N6-Propargyl-adenosine
N6-propargyl-adenosine (N6pA) can be used to measure de novo mRNA poly (A) tail synthesis in proliferating cells. N6pA is cell permeable and incorporates into nascent mRNA transcripts both transcriptionally by RNA polymerase I, II and III and posttranscription-ally by poly (A) polymerase instead of their natural analolg adenosine. The resulting ethynyl-functionalized RNA can subsequently be detected via Cu (I) -catalyzed click chemistry that offers the choice to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks or a fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging.
Product Specifications
Field of Research
Pharmacology & Drug Discovery
Purity
95% (HPLC)
Form
Solid; Color: white to off-white
Smiles
C1 (c2c (ncn1) n (cn2) [C@H]1[C@@H] ([C@@H] ([C@H] (O1) CO) O) O) NCC#C
Molecular Formula
C13H15N5O4
Molecular Weight
Theoretical MW: 305.29 g/mol; Detected MW: 305.11 g/mol
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
MRNA poly (A) tail synthesis monitoring. Spectroscopic Propertie: max 262 nm, 18.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .
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