What is the catalog number for 5-Ethynyl-dUTP (5-EdUTP)?

The catalog number for 5-Ethynyl-dUTP (5-EdUTP) is orb1734886.

What sizes are available for 5-Ethynyl-dUTP (5-EdUTP)?

5-Ethynyl-dUTP (5-EdUTP) is available in the following size(s): 10 ul.

Is 5-Ethynyl-dUTP (5-EdUTP) in stock?

5-Ethynyl-dUTP (5-EdUTP) is currently made to order / not held in immediate stock. Contact us for lead time.

5-Ethynyl-dUTP (5-EdUTP)

Product Specifications

Product Name Alternative

5-Ethynyl-2'-deoxyuridine 5'-triphosphate, Sodium salt

Concentration

100 mM-110 mM

Purity

95% (HPLC)

Form

Solution in water; Color: colorless to slightly yellow; pH: 7.5 0.5

Smiles

C1 (=O) [nH]c (=O) n (cc1C#C) [C@H]1C[C@H] (O) [C@H] (O1) COP (=O) (O) OP (=O) (O) OP (=O) (O) O

Molecular Formula

C11H15N2O14P3

Molecular Weight

Theoretical MW: 492.16 g/mol (free acid) ; Detected MW: 491.97 g/mol (free acid)

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible

Notes

For research use only.

Applications Notes

Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Ventexo-or KOD XL) . Incorporation into DNA by Primer Extension with family A polymerase Taqand family B polymerases (Pwo, Deep Vent exo-or KOD XL) . The resulting ethynyl-functionalized DNA can subsequently be processed via Cu (I) -catalyzed click chemistry that offers the choice: to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks; to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins. Spectroscopic Propertie: max 288 nm, 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .