5-Ethynyl-dUTP (5-EdUTP)
5-Ethynyl-dUTP (5-EdUTP)
Product Specifications
Product Name Alternative
5-Ethynyl-2'-deoxyuridine 5'-triphosphate, Sodium salt
Concentration
100 mM-110 mM
Purity
95% (HPLC)
Form
Solution in water; Color: colorless to slightly yellow; pH: 7.5 0.5
Smiles
C1 (=O) [nH]c (=O) n (cc1C#C) [C@H]1C[C@H] (O) [C@H] (O1) COP (=O) (O) OP (=O) (O) OP (=O) (O) O
Molecular Formula
C11H15N2O14P3
Molecular Weight
Theoretical MW: 492.16 g/mol (free acid) ; Detected MW: 491.97 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Incorporation into DNA by PCR with family B polymerases (Pwo, Deep Ventexo-or KOD XL) . Incorporation into DNA by Primer Extension with family A polymerase Taqand family B polymerases (Pwo, Deep Vent exo-or KOD XL) . The resulting ethynyl-functionalized DNA can subsequently be processed via Cu (I) -catalyzed click chemistry that offers the choice: to introduce a Biotin group (via Azides of Biotin) for subsequent purification tasks; to introduce fluorescent group (via Azides of fluorescent dyes) for subsequent microscopic imaging; to crosslink the DNA to Azide-functionalized biomolecules e.g.proteins. Spectroscopic Propertie: max 288 nm, 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
IBOTHRAFPRCK1MG 1 mg
Bovine Alpha Thrombin Purified FPRCK Active Site Blocked 1mg
Sign In for Pricing
View Details 