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5-Ethynyl-UTP (5-EUTP)

5-Ethynyl-UTP (5-EUTP)

Product Specifications

Product Name Alternative

5-Ethynyl-uridine-5'-triphosphate, Sodium salt

Concentration

100 mM-110 mM

Purity

95% (HPLC)

Form

Solution in water; Color: colorless to slightly yellow; pH: 7.5 0.5

Smiles

N1 ([C@@H]2O[C@H] (COP (=O) (OP (=O) (OP (=O) (O) O) O) O) [C@H] ([C@H]2O) O) c (=O) [nH]c (=O) c (c1) C#C

Molecular Formula

C11H15N2O15P3

Molecular Weight

Theoretical MW: 508.16 g/mol (free acid) ; Detected MW: 507.97 g/mol (free acid)

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible

Notes

For research use only.

Applications Notes

Incorporation into RNA by T7 RNA polymerase-mediated in vitro transcription. The resulting alkyne-functionalized RNA can subsequently be processed via Cu (I) -catalyzed (azide-alkyne) click chemistry that offers the choice: to introduce a Biotin group for subsequent purification tasks (via Azides of Biotin) ; to introduce fluorescent group for subsequent microscopic imaging (via Azides of fluorescent dyes) ; to crosslink the RNA to azide-functionalized biomolecules e.g.proteins. Spectroscopic Propertie: max 288 nm, 12.0 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .

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