8-Azido-ATP

Incorporation into RNA by 3'-End Labeling with yeast Poly (A) Polymerase (yPAP) . Identification of protein ATP-binding sites. The resulting azide-functionalized RNA can subsequently be processed via Cu (I) -free (azide-DBCO) or Cu (I) -catalyzed (azide-alkyne) click chemistry that offers the choice: to introduce a Biotin group for subsequent purification tasks (via DBCO-functionalized Biotin or Alkynes of Biotin, respectively) ; to introduce fluorescent group for subsequent microscopic imaging (DBCO-functionalized fluorescent dyes or Alkynes of fluorescent dyes, respectively) ; to crosslink the RNA to azide-or alkyne functionalized biomolecules e.g.proteins. Spectroscopic Propertie: λmax 281 nm, ε 13.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .