γ- (2-Azidoethyl) -ATP
Leeet al. reported a non-radioactive version of in vitro phos-phorylation were γ-[2-Azidoethyl]-ATP (compound 8) has been successfully used instead of γ-32P-modified ATP to phosphorylate GST-tagged recombinant p27kip1 with protein kinase cdk2. The phosphorylated, azide-modified protein substrate can sub-sequently be labeled with Alkynes of biotin or fluorescent dyes via Cu (I) -catalyzed Click-Chemistry or DBCO-containing biotin or fluorescent dyes via Cu (I) -free Click-Chemistry.
Product Specifications
Product Name Alternative
γ- (2-Azidoethyl) -adenosine-5'-triphosphate, Sodium salt
Concentration
10 mM-11 mM
Purity
≥ 95% (HPLC)
Form
Solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
Smiles
O[C@H]1[C@H] (n2cnc3c (N) ncnc23) O[C@H] (COP (=O) (O) OP (=O) (O) OP (=O) (O) OCCN=[N+]=[N-]) [C@H]1O
Molecular Formula
C12H19N8O13P3
Molecular Weight
Theoretical MW: 576.25 g/mol (free acid) ; Detected MW: 576.03 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
In vitro phosphorylation of recombinant proteins. Spectroscopic Propertie: λmax 259 nm, ε 15.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .
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