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Mant-AppNHp

(Mant-AMPPNP)

Product Specifications

CAS Number

85287-56-6

Product Name Alternative

(Mant-AMPPNP), 2'/3'-O- (N-Methyl-anthraniloyl) -adenosine-5'-[ (β, γ) -imido]-triphosphate, Triethylammonium salt

Concentration

10 mM-11 mM

Purity

≥ 90% (HPLC)

Form

Solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5

Smiles

OP (=O) (O) NP (=O) (O) OP (=O) (O) OC[C@H]1O[C@@H] (n2cnc3c (N) ncnc23) [C@H] (OC (=O) c2c (cccc2) NC) [C@@H]1O.OP (=O) (O) NP (=O) (O) OP (=O) (O) OC[C@H]1O[C@@H] (n2cnc3c (N) ncnc23) [C@H] (O) [C@@H]1OC (=O) c1c (cccc1) NC

Molecular Formula

C18H24N7O13P3

Molecular Weight

Theoretical MW: 639.35 g/mol (free acid) ; Detected MW: 639.06 g/mol (free acid)

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible

Notes

For research use only.

Applications Notes

Fluorescence stop-flow kinetics: helicase DnaB protein Displacement studies on TRP-MET-tyrosine kinase Nucleotide specific binding to membrane protein FeoB Inhibition of adenylyl cyclase X-ray studies of kinesin motors Agonistic ligand, mainly for nucleoside receptor A 1 Nucleosidephosphates stabilized against hydrolytic degradation can directly bind to nucleoside receptors. Spectroscopic Propertie: λmax 255/355 nm, ε 23.3/5.8 L mmol-1 cm-1, (Tris-HCl pH 7.5), λexc 355 nm, λem 448 nm. Specific Ligands: ATP-binding sites of serine protease, (10S) -myosin.

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