Aminoallyl-dUTP-ATTO-425
Aminoallyl-dUTP-ATTO425 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-ATTO425/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-ATTO425/80-70% dTTP (PCR), 30-50% Aminoallyl-dUTP-ATTO425/70-50% dTTP (Nick Translation) .
Product Specifications
Product Name Alternative
5- (3-Aminoallyl) -2'-deoxyuridine-5'-triphosphate, labeled with ATTO 425, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Solution in water; Color: green-yellow; pH: 7.5 ± 0.5
Smiles
Molecular Formula
C34H45N4O19P3
Molecular Weight
Theoretical MW: 906.66 g/mol (free acid) ; Detected MW: 906.16 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Frequently Asked Questions
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