Aminoallyl-dUTP-ATTO-643
Aminoallyl-dUTP-643 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. ATTO 643 is a hydrophilic dye with excellent photostability compared to fluorescein. Recommended Aminoallyl-dUTP-643/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-643/70-50% dTTP.
Product Specifications
Product Name Alternative
5- (3-Aminoallyl) -2'-deoxyuridine-5'-triphosphate, labeled with ATTO 643, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Filtered solution (30 kDa) in 10 mM Tris-HCl; Color: blue; pH: 7.5 ± 0.5
Smiles
C (/C=C/c1cn (c (=O) [nH]c1=O) [C@H]1C[C@@H] ([C@H] (O1) COP (=O) (O) OP (=O) (O) OP (=O) (O) O) O) N*
Molecular Formula
C12H19N3O14P3-ATTO 643
Molecular Weight
Theoretical MW: 1340.4 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Frequently Asked Questions
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