What is the catalog number for Aminoallyl-dUTP-ATTO-643?

The catalog number for Aminoallyl-dUTP-ATTO-643 is orb1733886.

What sizes are available for Aminoallyl-dUTP-ATTO-643?

Aminoallyl-dUTP-ATTO-643 is available in the following size(s): 10 ul.

Is Aminoallyl-dUTP-ATTO-643 in stock?

Aminoallyl-dUTP-ATTO-643 is currently made to order / not held in immediate stock. Contact us for lead time.

Aminoallyl-dUTP-ATTO-643

Aminoallyl-dUTP-643 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. ATTO 643 is a hydrophilic dye with excellent photostability compared to fluorescein. Recommended Aminoallyl-dUTP-643/dTTP ratio for PCR and Nick Translation: 30-50% Aminoallyl-dUTP-643/70-50% dTTP.

Product Specifications

Product Name Alternative

5- (3-Aminoallyl) -2'-deoxyuridine-5'-triphosphate, labeled with ATTO 643, Triethylammonium salt

Concentration

1.0 mM-1.1 mM

Purity

≥ 95% (HPLC)

Form

Filtered solution (30 kDa) in 10 mM Tris-HCl; Color: blue; pH: 7.5 ± 0.5

Smiles

C (/C=C/c1cn (c (=O) [nH]c1=O) [C@H]1C[C@@H] ([C@H] (O1) COP (=O) (O) OP (=O) (O) OP (=O) (O) O) O) N*

Molecular Formula

C12H19N3O14P3-ATTO 643

Molecular Weight

Theoretical MW: 1340.4 g/mol (free acid)

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible

Notes

For research use only.

Applications Notes

Incorporation into DNA/cDNA by: PCR with Taq polymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 643 nm, λem 665 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) .