Rhodamine-12-dUTP
Rhodamine-12-dUTP is recommended for direct enzymatic labeling of DNA/cDNA by Nick Translation. It is incorporated as substi-tute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Rhodamine-12-dUTP/dTTP ratio for Nick Trans-lation: 30-50% Rhodamine-12-dUTP/70-50% dTTP.
Product Specifications
Product Name Alternative
Rhodamine-X- (5-aminoallyl) -dUTP, Rho-green-X-dUTP, RhG-dUTP, R110-dUTP
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Solution in water; Color: orange; pH: 7.5 ± 0.5
Smiles
Molecular Formula
C39H43N6O19P3
Molecular Weight
Theoretical MW: 992.71 g/mol (free acid) ; Detected MW: 992.18 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Frequently Asked Questions
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