Aminoallyl-dUTP-Texas Red
Aminoallyl-dUTP-Texas Red is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling.Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-Texas Red/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-Texas Red/80-70% dTTP (PCR), 50% Aminoallyl-dUTP-Texas Red/50% dTTP (Nick Translation) .
Product Specifications
Product Name Alternative
TexasRed-12-dUTP, 5- (3-Aminoallyl) -2'-deoxyuridine-5'-triphosphate, labeled with Texas Red, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Filtered solution (30 kDa) in 10 mM Tris-HCl; Color: red-violet; pH: 7.5 ± 0.5
Smiles
Molecular Formula
C49H59N6O21P3S2
Molecular Weight
Theoretical MW: 1225.07 g/mol (free acid) ; Detected MW: 1224.24 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Frequently Asked Questions
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