Aminoallyl-dUTP-XX-Cy5
Aminoallyl-dUTP-XX-Cy5 is recommended for direct enzymatic label-ing of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dTTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of uridine. Recommended Aminoallyl-dUTP-XX-Cy5/dTTP ratio for PCR and Nick Translation: 20-30% Aminoallyl-dUTP-XX-Cy5/80-70% dTTP.
Product Specifications
Product Name Alternative
5- (3-Aminoallyl) -2'-deoxyuridine-5'-triphosphate, labeled with Cy5, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Filtered solution (30 kDa) in 10 mM Tris-HCl; Color: blue; pH: 7.5 ± 0.5
Smiles
Molecular Formula
C57H80N7O23P3S2
Molecular Weight
Theoretical MW: 1388.33 g/mol (free acid) ; Detected MW: 1387.40 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
ACTH (Adrenocorticotrophic Hormone) (AH26), CF740 conjugate, 0.1mg/mL
Cytokeratin, LMW (AE-1), CF568 conjugate, 0.1mg/mL
Calponin-1 (CNN1/832), CF568 conjugate, 0.1mg/mL
