What is the catalog number for 5-Propargylamino-dCTP-Cy3?

The catalog number for 5-Propargylamino-dCTP-Cy3 is orb1733850.

What sizes are available for 5-Propargylamino-dCTP-Cy3?

5-Propargylamino-dCTP-Cy3 is available in the following size(s): 10 ul.

Is 5-Propargylamino-dCTP-Cy3 in stock?

5-Propargylamino-dCTP-Cy3 is currently made to order / not held in immediate stock. Contact us for lead time.

5-Propargylamino-dCTP-Cy3

5-Propargylamino-dCTP-Cy3 is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incor-porated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-Cy3/dCTP ratio for PCR and Nick Translation: 30-50% Propargylamino-dCTP-Cy3/70-50% dCTP.

Product Specifications

Product Name Alternative

5-Propargylamino-2'-deoxycytidine-5'-triphosphate, labeled with Cy3, Triethylammonium salt

Concentration

1.0 mM-1.1 mM

Purity

≥ 95% (HPLC)

Form

Solution in 10 mM Tris-HCl; Color: pink; pH: 7.5 ± 0.5

Smiles

N (CC#Cc1cn (c (=O) nc1N) [C@@H]1O[C@H] (COP (=O) (O) OP (=O) (O) OP (=O) (O) O) [C@H] (C1) O) C (=O) CCCCCN1c2ccc (S (=O) (=O) [O-]) cc2C (C) (C) /C/1=C\C=C\C1=* (c2ccc (cc2C1 (C) C) S (=O) (=O) O) CC

Molecular Formula

C43H55N6O20P3S2

Molecular Weight

Theoretical MW: 1132.97 g/mol (free acid) ; Detected MW: 1132.21 g/mol (free acid)

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible

Notes

For research use only.

Applications Notes

Incorporation into DNA/cDNA by: Primer Extension with Klenow fragment; PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 550 nm, λem 570 nm, ε 150.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) .