What is the catalog number for Fluorescein-12-dCTP?

The catalog number for Fluorescein-12-dCTP is orb1733848.

What sizes are available for Fluorescein-12-dCTP?

Fluorescein-12-dCTP is available in the following size(s): 10 ul.

Is Fluorescein-12-dCTP in stock?

Fluorescein-12-dCTP is currently made to order / not held in immediate stock. Contact us for lead time.

Fluorescein-12-dCTP

Fluorescein-12-dCTP is recommended for direct enzymatic labeling of DNA/cDNA e.g. by PCR and Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Fluorescein-12-dCTP/dCTP ratio for PCR and Nick Translation: 30-50% Fluorescein-12-dCTP-/70-50% dCTP.

Product Specifications

Product Name Alternative

Fluorescein-X-5-Propargylamino-dCTP, 5/6-Fluorescein-X-5-propargylamino-2'-deoxycytidine-5'-triphosphate, Triethylammonium salt

Concentration

1.0 mM-1.1 mM

Purity

≥ 95% (HPLC)

Form

Solution in 10 mM Tris-HCl; Color: yellow-orange; pH: 7.5 ± 0.5

Smiles

[C@@H]1 (C[C@@H] (O[C@@H]1COP (=O) (OP (=O) (OP (=O) (O) O) O) O) n1c (=O) nc (c (c1) C#CCNC (=O) CCCCCNC (=O) c1ccc (c (c1) c1c2c (oc3c1ccc (=O) c3) cc (cc2) O) C (=O) O) N) O.[C@@H]1 (C[C@@H] (O[C@@H]1COP (=O) (OP (=O) (OP (=O) (O) O) O) O) n1c (=O) nc (c (c1) C#CCNC (=O) CCCCCNC (=O) c1ccc (c (c1) C (=O) O) c1c2c (oc3c1ccc (=O) c3) cc (cc2) O) N) O

Molecular Formula

C39H40N5O20P3

Molecular Weight

Theoretical MW: 991.68 g/mol; Detected MW: 991.15 g/mol

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible

Notes

For research use only.

Applications Notes

Incorporation into DNA/cDNA by: PCR with Taqpolymerasein-house data; Nick Translation with DNAse I/DNA Polymerase Iin-house data. Spectroscopic Propertie: λexc 492 nm, λem 517 nm, ε 83.0 L mmol-1 cm-1 (Tris-HCl pH 7.5) .