5-Hydroxymethyl-dCTP
5-Hydroxymethylated DNA probes can be used as sequencing control or for pull-down of 5-hmC binding proteins from cellular lysate.
Product Specifications
CAS Number
7226-77-9
Product Name Alternative
5-hmdCTP, 5-Hydroxymethyl-2'-deoxycytidine-5'-triphosphate, Sodium salt
Concentration
100 mM-110 mM
Purity
≥ 95% (HPLC), < 0.5% dCTP (HPLC)
Form
Solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
Smiles
Nc1c (cn (c (=O) n1) [C@@H]1O[C@H] (COP (=O) (OP (=O) (OP (=O) (O) O) O) O) [C@@H] (O) C1) CO
Molecular Formula
C10H18N3O14P3
Molecular Weight
Theoretical MW: 497.18 g/mol (free acid) ; Detected MW: 497.00 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Incorporation into DNA by-PCR with Taqpolymerasein-house data. Spectroscopic Propertie: λmax 275 nm, ε 7.8 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .
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