AP3G (A cap) - Solution
GP3A, GpppA, G (5') ppp (5') A, A (5') ppp (5') G
Product Specifications
Product Name Alternative
GP3A, GpppA, G (5') ppp (5') A, A (5') ppp (5') G, P1- (5'-Adenosyl) P3- (5'-guanosyl) triphosphate, Sodium salt
Concentration
100-110 mM
Purity
≥ 95% (HPLC)
Form
Solution in water; Color: colorless to slightly yellow; pH: 7.5 ± 0.5
Smiles
O=c1[nH]c (N) nc2c1ncn2[C@@H]1O[C@H] (COP (=O) (OP (=O) (OP (=O) (OC[C@@H]2[C@H] ([C@H] ([C@@H] (O2) n2c3c (nc2) c (ncn3) N) O) O) O) O) O) [C@@H] (O) [C@H]1O
Molecular Formula
C20H27N10O17P3
Molecular Weight
Theoretical MW: 772.41 g/mol (free acid) ; Detected MW: 772.08 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Synthesis of mRNA with a non-functional cap analog (ApppG) to estimate the level of cap-independent translation; Investigation of stress related (Near UV and oxidation) product formation in bacteria. Spectroscopic Propertie: λ 259 nm, ε 27.0 L mmol-1 cm-1 (Tris-HCl pH, 7.5) .
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