Aminoallyl-dUTP-XX-IR750
Recommended Aminoallyl-dUTP-XX-IR750/dTTP ratio for PCR: 20-30% Aminoallyl-dUTP-XX-IR750/80%-70% dTTP.
Product Specifications
Product Name Alternative
5- (3-Aminoallyl) -2'-deoxyuridine-5'-triphosphate, labeled with IR750, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Solution in 10 mM Tris-HCl; Color: dark green; pH: 7.5 ± 0.5
Smiles
P (=O) (OP (=O) (O) O) (O) OP (=O) (O) OCC1OC (C[C@@H]1O) n1c (=O) [nH]c (=O) c (c1) /C=C/CNC (=O) CCCCCNC (=O) CCCCCNC (=O) CCCCc1ccc (cc1) C1=C (CCC/C/1=C\C=C/1\N (c2c (C1 (C) C) cc (cc2) S (=O) (=O) O) CCCS (=O) (=O) O) /C=C/C1=[N+] (c2c (C1 (C) C) cc (cc2) S (=O) (=O) [O-]) CCCS (=O) (=O) O
Molecular Formula
C71H96N7O29P3S4
Molecular Weight
Theoretical MW: 1732.73 g/mol (free acid) ; Detected MW: 1731.43 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Incorporation into DNA/cDNA by-PCR with Taqpolymerasein-house data. Spectroscopic Propertie: λexc 753 nm, λem 775 nm, ε 255.0 L mmol-1, cm-1 (Tris-HCl pH 7.5) .
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