HighYield T7 IR750 RNA Labeling Kit (UTP-based)

HighYield T7 IR750 RNA Labeling Kit (UTP-based) is designed to pro-duce randomly IR750-modified RNA probes via in vitro transcription. Such probes are ideally suited for in situ hybridization and North-ern Blot experiments. IR750 (a structural analog of IRDye750) is hydrophilic dye ideally suited for infrared fluorescence spectroscopy. UTP-PEG5-IR750 is efficiently incorporated into RNA as substi-tute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase Mix. 35% UTP-PEG5-IR750 substitution typically results in an optimal balance between reaction and labeling efficiency. Individual optimization of UTP-PEG5-IR750/UTP ratio however, can easily be achieved with the single nucleotide format. The resulting IR750-modified RNA probe can subsequently be detected by fluorescence spectroscopy. The kit contains sufficient reagents for 20 labeling reactions of 20 μl each (35% UTP-PEG5-IR750 substitution, 2.5 mM ATP, GTP, CTP, 0.2 mM UTP, 0.1 mM UTP-PEG5-IR750) .