Biotinylated Human NKG2C&CD94 Protein, His, Avitag™ (MALS verified)

CD94 plays a role as a receptor for the recognition of MHC class I HLA-E molecules by NK cells and some cytotoxic T-cells. KLRD1 (CD94) is an antigen preferentially expressed on NK cells and is classified as a type II membrane protein because it has an external C terminus. NKG2C/CD159c is used as a receptor for NK cells and some cytotoxic T cells to recognize MHC class I HLA-E molecules. CD94 pairs with the NKG2 molecule as a heterodimer. The CD94/NKG2 complex, on the surface of natural killer cells interacts with Human Leukocyte Antigen (HLA) -E on target cells.

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™) . The protein has a calculated MW of 34.3 kDa. The protein migrates as 45-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Immobilized Biotinylated Human NKG2C&CD94, His,Avitag (Cat. No. NC4-H82E3) at 5 μg/mL (100 μL/well) can bind PE-Labeled Human HLA-E*01:03&B2M&CMV UL40 (VMAPRTVLL) Tetramer Protein (Cat. No. HLU-HP2H5) with a linear range of 0.01-1.25 μg/mL (QC tested).