Biotin-11-dUTP

Biotin-11-dUTP is enzymatically incorporated into DNA/cDNA as sub-stitute for its natural counterpart dTTP. The resulting Biotin-labeled DNA/cDNA probes are subsequently detected using streptavidin conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP), a fluorescent dye or agarose/magnetic beads. Optimal sub-strate properties and thus labeling efficiency as well as an efficient detection of the Biotin moiety is ensured by a 11-atom linker attached to the C5 position of uridine. Recommended Biotin-11-dUTP/dTTP ratio for PCR and Nick Translation: 50% Biotin-11-dUTP/50% dTTP.

Product Specifications

Product Name Alternative

Biotin-X- (5-aminoallyl) -dUTP, γ-[N- (Biotin-6-amino-hexanoyl) ]- (5-aminoallyl) -2'-deoxyuridine-5'-triphosphate, Triethylammonium salt

Concentration

1.0 mM-1.1 mM

Purity

≥ 95% (HPLC)

Form

Filtered solution (30 kDa) in 10 mM Tris-HCl; Color: colorless to slightly yellow; pH: 7.5 ± 0.5

Smiles

C1[C@H]2[C@@H] ([C@@H] (S1) CCCCC (=O) NCCCCCC (=O) NC/C=C/c1c (=O) [nH]c (=O) n ([C@H]3C[C@@H] ([C@H] (O3) COP (=O) (OP (=O) (OP (=O) (O) O) O) O) O) c1) NC (=O) N2

Molecular Formula

C28H45N6O17P3S

Molecular Weight

Theoretical MW: 862.67 g/mol (free acid) ; Detected MW: 862.18 g/mol (free acid)

Storage Conditions

Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible

Notes

For research use only.

Applications Notes

Incorporation into DNA/cDNA by: PCR with Taqpolymerase & in-house data; Nick Translation with DNAse I/DNA Polymerase I & in-house data; Primer Extension with Klenow 3'-5' exo-; 3'-End Labeling with Terminal deoxynucleotidyl Transferase (TdT) ; Reverse Transcription with MMLV Reverse Transcriptase. Spectroscopic Propertie: λmax 240/289 nm, ε 10.7/7.1 L mmol-1 cm-1, (Tris-HCl pH 7.5) .