5-Propargylamino-dCTP - Solution
Propargylamino-dCTP is recommended for two-step labeling of DNA e.g. by PCR. It is enzymatically incorporated into DNA as substitute for its natural counterpart dCTP. The resulting Amine-functionalized DNA can subsequently be labeled via the classic Amine/NHS Ester reaction that offers the choice: to introduce a Biotin group (via NHS Ester of Biotin) for subse-quent purification tasks; to introduce fluorescent group (via NHS Ester of fluorescent dyes) for subsequent microscopic imaging.
Product Specifications
CAS Number
115899-39-3
Product Name Alternative
5-Propargylamino-2'-deoxycytidine-5'-triphosphate, Sodium salt
Concentration
100 mM-110 mM
Purity
≥ 93% (HPLC)
Form
Solution in water; Color: slightly yellow; pH: 7.5 ± 0.5
Smiles
NCC#Cc1cn (c (=O) nc1N) [C@@H]1O[C@H] (COP (=O) (O) OP (=O) (O) OP (=O) (O) O) [C@@H] (O) C1
Molecular Formula
C12H19N4O13P3
Molecular Weight
Theoretical MW: 520.22 g/mol (free acid) ; Detected MW: 520.02 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Incorporation into DNA by PCR with Taqpolymerasein-house data. Spectroscopic Propertie: λmax 294 nm, ε 9.3 L mmol-1 cm-1 (Tris-HCl, pH 7.5) .
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