DDAO Phosphate
DDAO-phosphate is a long-wavelength, phosphatase substrate for both solution-based assays and the amplified detection of specific targets on blots. Hydrolysis of the phosphate bond yields a brightly red-fluorescent product with absorption/emission maxima of 646/659 nm that is efficiently excited by the 633 nm spectral line of the He-Ne laser. The shift of emission maxima between DDAO-phosphate and the dephosphorylated product is larger than 200 nm and permits both species to be easily distinguished. DDAO phosphate has good water solubility, a low KM and a high turnover rate.
Product Specifications
CAS Number
500883-59-0
Product Name Alternative
9H- (1,3-Dichloro-9,9-dimethylacridin-2-one-7-yl) phosphate, Bis (triethylammonium) salt
Purity
> 95% (HPLC)
Form
Solid
Smiles
C1cc2c (cc1OP (=O) (O) O) C (C1=C (C (=O) C (=CC1=N2) Cl) Cl) (C) C
Molecular Formula
C15H12Cl2NO5P
Molecular Weight
Theoretical MW: 388.14 g/mol (free acid) ; Detected MW: 386.98 g/mol (free acid)
Storage Conditions
Store at -20 °C. store dark
Notes
For research use only.
Applications Notes
Spectroscopic Propertie: λexc 477 nm, λem 628 nm, ε 26.0 L mmol-1 cm-1 (50 mM Potassium Phosphate pH 7.0) .
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