5-Propargylamino-dCTP-ATTO-550
5-Propargylamino-dCTP-ATTO-550 is recommended for direct enzy-matic labeling of DNA/cDNA by Nick Translation. It is incorporated as substitute for its natural counterpart dCTP. The resulting Dye-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. Optimal substrate properties and thus labeling efficiency is ensured by an optimized linker attached to the C5 position of cytidine. Recommended Propargylamino-dCTP-ATTO-550/dCTP ratio for Nick Translation: 30-50% Propargylamino-dCTP-ATTO-550/70-50% dCTP.
Product Specifications
Product Name Alternative
5-Propargylamino-2'-deoxycytidine-5'-triphosphate, labeled with ATTO 550, Triethylammonium salt
Concentration
1.0 mM-1.1 mM
Purity
≥ 95% (HPLC)
Form
Filtered solution (30 kDa) in 10 mM Tris-HCl; Color: red-violet; pH: 7.5 ± 0.5
Smiles
Molecular Formula
C49H60N7O16P3
Molecular Weight
Theoretical MW: 1095.97 g/mol (free acid) ; Detected MW: 1095.33 g/mol (free acid)
Storage Conditions
Store at -20 °C. Short term exposure (up to 1 week cumulative) to ambient temperature possible
Notes
For research use only.
Applications Notes
Frequently Asked Questions
Explore Other Products
Browse additional items from our catalog
IgA Secretory Component (SC05), CF488A conjugate, 0.1mg/mL
Mucin 1 / EMA / Episialin / CD227 (VU-2G7), CF405S conjugate, 0.1mg/mL
Fibronectin (HFN7.1), CF594 conjugate, 0.1mg/mL
