Anti-VGlut1 Transporter Antibody

Mouse Monoclonal Antibody specific to VGlut1 Transporter

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

VGluT1, Brain-specific Na(+-dependent inorganic phosphate cotransporter, Solute carrier family 17 member 7

Gene Name

Slc17a7

NCBI Gene ID

UniProt

Q62634

Cellular Locus

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Membrane, Cell junction, synapse, synaptosome

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 493-560 (cytoplasmic C-terminus) of rat VGlut1 (accession no. NP_446311).

Target Antigen

Vesicular glutamate transporter 1

Target

VGlut1 Transporter

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. {PubMed:10938000, PubMed:11001057, PubMed:11698619, PubMed:11698620, PubMed:18080752, PubMed:8202535}.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of ~52kDa is detected. Immunohistochemistry: use at 1-5ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: rat brain lysate.

Storage Conditions

This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat VGlut1. It does not cross-react with VGlut2.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

VGlut1 is expressed in a subset of glutamate neurons where it transports glutamate into native synaptic vesicles from the brain. Glutamate transport by VGlut1 is saturated with a K(m) of approximately 2mM. Whereas plasma membrane glutamate transporters recognize both aspartate and glutamate as substrates, VGlut1 recognizes only glutamate.