Mouse Anti-Human CD33-LE/AF

The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2- 8 °C. <br>The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 °C. <br>The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 °C. Do not freeze! <br>The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 °C. Do not freeze! <br>The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 °C. <br>With the exception of the low endotoxin, azide-free product, the vials should be stored at 2-8 °C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Human CD33<br>CD33 is a 67 kDa type I transmembrane glycoprotein and a member of the sialoadhesin family of cell surface receptors. It is absent from pluripotent stem cells but appears on myelomonocytic precursors after CD34. It then continues to be expressed on both the myeloid and monocyte lineages, although it is absent on granulocytes. While it has been reported that CD33 can function as a sialic acid-dependent cell adhesion molecule, cells expressing CD33 require desialylation before they can bind cells bearing the appropriate sialoglycoconjugates. This suggests that inhibitory cis interactions may regulate or block any adhesion function.1-4

[CD33 antigen (gp67); Myeloid cell surface antigen CD33; myeloid cell surface antigen CD33; gp67; CD33 antigen (gp67); sialic acid binding Ig-like lectin 3; sialic acid-binding Ig-like lectin 3; CD33 molecule; Sialic acid-binding Ig-like lectin 3; Siglec-3; gp67]