Mouse Anti-Chicken Bu-1-BIOT

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 °C. <br> The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C. <br> The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C. <br> The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 °C. Do not freeze! <br> Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

Chicken Bu-1 (chB6), Mr 70-75 kDa<br> Chicken Bu-1, a product of the Bu-1a and Bu-1b alleles, is a type I transmembrane glycosylated, disulfide-linked homodimer. MAb AV20 recognizes a monomorphic determinant on the Bu-1 B cellassociated alloantigens of both RPL 6(3) (Bu-1a) and 7(2) (Bu-1b) lines of inbred chickens. The antibody also identifies B cells in partially inbred birds. Bu-1 is found on 85-90% of bursal cells, 2-8% of thymocytes, 15-27% of spleen cells, and 2-18% of peripheral blood cells. It is also expressed on subsets of macrophages and monocytes, but not on granulocytes, erythrocytes or thrombocytes.1-2 MAb AV20 does not cross react with turkey cells when analyzed by immunofluorescent staining and flow cytometry.3