Mouse Anti-Chicken MHC Class I-BIOT

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 °C. <br>The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C. <br>The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C. <br>The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 °C. Do not freeze! <br>Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

A monomorphic epitope on avian MHC Class I molecules (Mr ~ 68 kDa)<br>Like their mammalian counterparts, avian MHC Class I molecules (also known as B-F antigens) consist of a highly polymorphic alpha-chain (Mr ~ 44 kDa) non-covalently bound to the invariant beta 2-microglobulin subunit (Mr ~ 12 kDa). MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.1-3 MAb F21-2 also reacts with turkey MHC Class I.4