Mouse Anti-Chicken CD45-APC

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 °C. <br>The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C. <br>The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C. <br>The R-phycoerythrin (R-PE) and allophycocyanin (APC) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 °C. Do not freeze! <br>The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 °C. Do not freeze! <br>Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

Chicken CD45 (Mr 190 to 215-kDa).<br>Chicken CD45 is a transmembrane glycoprotein expressed on all leukocytes. B lineage cells in the bursa of Fabricius express the 215 kDa isoform while T lineage cells bear the 190 kDa variant. These high molecular weight molecules have intrinsic phosphotyrosine phosphatase activity characteristic of mammalian CD45.1 Levels of CD45 expression detected by MAb LT40 in the avian thymus are heterogeneous with approximately 90% of thymocytes expressing fourfold higher levels of surface CD45 (CD45hi) than do the remaining 10% (CD45lo) of thymocytes. The CD45lo population contains exclusively thymocytes with the CD3-CD4-CD8lo phenotype characteristic of the immediate precursors to the CD3- CD4+CD8+ thymic population which are CD45hi. This shift from low to high levels of surface CD45 expression is concomitant with the transition from CD4-CD8lo to CD4+CD8+ and before the expression of CD3. The ability of MAb LT40 to discriminate this CD45lo subpopulation provides a suitable marker for the CD3-CD4-CD8lo immediate precursors to the CD3-CD4+CD8+ thymocytes.1

[CD45; Receptor-type tyrosine-protein phosphatase C; receptor-type tyrosine-protein phosphatase C; CD45 antigen; T200 glycoprotein; OTTHUMP00000033813; OTTHUMP00000033816; OTTHUMP00000033817; OTTHUMP00000038574; leukocyte common antigen; leukocyte-common antigen; T200 leukocyte common antigen; protein tyrosine phosphatase, receptor type, c polypeptide; protein tyrosine phosphatase, receptor type, C; Leukocyte common antigen; L-CA; T200]