Mouse Anti-Chicken CD28-BIOT

The purified antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added; store at 2-8 °C. <br>The FITC conjugate is supplied as 0.5 mg in 1.0 mL of phosphate buffered saline, pH 7.4, containing 0.1% sodium azide as preservative (PBS/NaN3); store at 2-8 °C. <br>The BIOT conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3; store at 2-8 °C. <br>The R-PE conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent; store at 2-8 °C. <br>All products should be stored at 2-8 °C. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Each reagent is stable for the period shown on the bottle label if stored as directed.

Chicken CD28, a monomeric molecule of Mr 40-44 kDa with 50% amino acid sequence identity with mammalian CD28<br>Chicken CD28 antigen is a type I transmembrane monomeric glycoprotein which does not contain the cysteine residues that form disulfide-linked covalent homodimers found in the mammalian counterpart. This may reduce the avidity of binding to individual ligand molecules expected to be present on Ag presenting cells. Peripheral gamma delta + T cells are CD28-negative.1 Monoclonal antibodies against CD28 have a costimulatory effect on T cells stimulated by phorbol myristate acetate (PMA), concanavalin A or MAbs against the avian T cell receptor (TCR). Both V beta 1- and V beta 2-expressing cells respond equally well to stimulation with anti-CD28 in combination with PMA. These responses are resistant to cyclosporin A, but inhibited by herbimycin A, suggesting that CD28 employs a signalling pathway at least partly distinct from that triggered by TCR/CD3.2 MAb AV7 also reacts with turkey CD28 as demonstrated by flow cytometry and immunoprecipitation/Western blotting.3