Mouse Anti-Mouse H-2Dd-BIOT

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 °C. <br>The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C. <br>The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C. <br>The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 °C. Do not freeze! <br>Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

A conformational epitope on the alpha1 and alpha2 chains of mouse H-2Dd class I alloantigen<br>MAb 34-5-8S reacts with a conformational epitope on H-2Dd MHC Class I found on the N-terminal domains of alpha1 and alpha2 chains when complexed with beta 2-microglobulin.2 The antibody does not react with H-2Dd alpha chains synthesized in vitro.3 Weak cross-reactivity with cells from mice of the H-2b, H-2q, and H- 2s haplotypes has been observed by flow cytometric analysis.4 Reactivity with cells from mice of the H-2f, H-2k, H-2p, and H-2r haplotypes has not been observed.2 MAb 34-5-8S has been reported to block the recognition of H-2Dd by Ly-49A+, Ly-49C+, and Ly-49G2+ natural killer cells.5-9