Mouse Anti-Mouse H-2Kd/H-2Dd-FITC

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 °C. <br>The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C. <br>The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C. <br>The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 °C. Do not freeze! <br>Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

An epitope in the alpha 3 domain that is common to H-2Kd and H-2Dd<br>The "classical" MHC Class I molecules are histocompatibility antigens encoded by the H-2 gene complex and consist of heterodimers of highly polymorphic alpha chains noncovalently associated with the invariant beta 2-microglobulin.3,4 These antigens are expressed on most nucleated cells but expression varies on different cell types. MHC Class I molecules present endogenously synthesized peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells.5 MHC Class I antigens expressed on thymic epithelial cells regulate the positive and negative selection of CD8+ T cells during T cell ontogeny.3,6 The monoclonal antibody 34-1-2S binds to a common determinant in the alpha 3 domains of H-2Kd and H-2Dd in the presence or absence of beta 2 microglobulin.7,8 It cross reacts with the alpha 3 domain of H-2Kb.1,2