Hamster Anti-Mouse Ly-49C/F/H/I-BIOT

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 °C. <br>The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C. <br>The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C. <br>The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 °C. Do not freeze! <br>Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label if stored as directed.

An epitope common to the Ly49C, Ly49F, Ly49H and Ly49I allelic/splice variants<br>The monoclonal antibody 14B11 reacts with murine Ly49C, Ly49F, Ly49H and Ly49I, members of the lectin-like homodimeric Ly49 family of cell surface receptors.1 Mouse Ly49 receptors exhibit allelic specificity for MHC class I Ia molecules and are thought to serve to prevent natural killer (NK) cells from attacking normal cells, while allowing them to attack infected or transformed cells in which class I molecules have been down-regulated.2 These inhibitory receptors are also expressed on a subpopulation of mouse CD8+ T cells. The 14B11 antibody stains ~ 90% of NK1.1+CD3- splenocytes, and ~ 6% of CD8+ spleen cells from C57BL/6 mice.1 The antibody also stains 30-80% of NK1.1+CD3- splenocytes in other mouse strains tested (BALB/c, C3H, 129, SJL, AKR/J).