Rat Anti-Mouse CD25/IL-2Ra-SPRD

The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 °C. <br>The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C. <br>The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2-8 °C. <br>The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 °C. Do not freeze! <br>The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 °C. Do not freeze! <br>The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 7 (R-PE-CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 °C. Do not freeze! <br>The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 °C. <br>Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide-free product at -20 °C immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.

Low affinity alpha chain IL-2 receptor, Mr 55 kDa (IL-2R alpha)<br>The IL-2 receptor (IL-2R) exists in three alternative forms made up from the individual components of CD25, CD122, and CD132. CD25 represents the low affinity alpha chain of the IL-2R, a type I transmembrane glycoprotein containing two CCP domains. It is rich in O-linked carbohydrates and has a short cytoplasmic tail.^14 CD25 is expressed on activated T cells, B cells, NK cells and monocytes of all mouse strains tested.^2, 3, 15 Expression of CD25 on activated T lymphocytes is transitory and endogenously regulated.^4 CD25 is expressed on precursor B cells in bone marrow. Its expression is initiated by functional rearrangement and expression of IgM heavy chain genes and is down-regulated when immature B cells mature and express IgD.^5 It is expressed at a higher level on CD4^+CD8^+ thymocytes.^5, 6 It is also expressed on cultured epidermal Langerhans cells.^7 The biochemical features of murine CD25 have been characterized in detail.^8-10 The 3C7 monoclonal antibody reacts with an epitope of CD25 which is distinct from that recognized by clone 7D4.^2, 11, 12 3C7, used in combination with 7D4 in culture, results in higher levels of inhibition of proliferation driven by IL-2 and generation of alloreactive CTL than either of these monoclonal antibodies alone.^2, 13