Caspase 4 (p10, Cleaved-Ala290) rabbit pAb

Catalytic activity: Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|- but also cleaves at Asp-Glu-Val-Asp-|-. function: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. PTM: The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8. similarity: Belongs to the peptidase C14A family. similarity: Contains 1 CARD domain. subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit. tissue specificity: Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.

Product Specifications

Background

Catalytic activity:Strict requirement for Asp at the P1 position. It has a preferred cleavage sequence of Tyr-Val-Ala-Asp-|- but also cleaves at Asp-Glu-Val-Asp-|-., function:Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1., PTM:The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8., similarity:Belongs to the peptidase C14A family., similarity:Contains 1 CARD domain., subunit:Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit., tissue specificity:Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain.

UniProt

P49662

Swiss Prot

P49662

Reactivity

Human

Immunogen

Synthesized peptide derived from human Caspase 4 (p10, Cleaved-Ala290)

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

WB 1:1000-2000 ELISA 1:5000-20000

Molecular Weight

10 42kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

10 42kD

Fragment

IgG

Subcellular Location

Cytoplasm, cytosol . Endoplasmic reticulum membrane ; Peripheral membrane protein ; Cytoplasmic side . Mitochondrion . Inflammasome . Secreted . Predominantly localizes to the endoplasmic reticulum (ER) . Association with the ER membrane requires TMEM214 (PubMed:15123740) . Released in the extracellular milieu by keratinocytes following UVB irradiation (PubMed:22246630) . .