IgG2b Negative Control (FITC)

May be stored at 4 °C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 °C. Aliquots are stable for at least 6 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Can be used as a negative control for direct FITC immunofluorescence staining (eg. Flow Cytometry) when using mouse monoclonal antibodies to human cell surface antigens of isotype IgG2b. This enables an estimation of non-specific binding of mouse monoclonal antibodies to cell surface components in peripheral blood and tissue.