Human M-CSF Protein, His tag (Animal-Free)

Macrophage Colony Stimulating Factor (M-CSF) is a 18.54 kDa member of hematopoietic Growth Factors with 159 amino acid residues. M-CSF produced by osteoblasts and osteoblast precursors. M-CSF stimulates the growth and differentiation of the monocyte lineage, and promotes the survival, proliferation, and functions of mature monocytes/macrophages.

Product Specifications

Background

Macrophage Colony Stimulating Factor (M-CSF) produced by osteoblasts and osteoblast precursors. M-CSF stimulates the growth and differentiation of the monocyte lineage, and promotes the survival, proliferation, and functions of mature monocytes/macrophages

Product Name Alternative

CSF-1, MGI-IM

Accession Number

P09603

Tag

M-CSF

Type

Cytokines and proteins

Applications

His tag

Purity

>98% as determined by SDS-PAGE.

Activity

Measured by its ability to induce proliferation in NFS-60 cells.The ED50 for this effect is <1 ng/mL.The specific activity of recombinant human M-CSF is approximately > 2.5x 108IU/mg

Molecular Weight

19.76 kDa

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Features & Benefits

Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA) . Please prevent freeze-thaw cycles

Protein Length

The recombinant Human M-CSF consists of 158 amino acids and predicts a molecular mass of 19.76 kDa

Formulation

Lyophilized from PBS, pH 7.4

Applications Notes

Human

Other Product Names

CSF-1, MGI-IM

Recommended Usage

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration not less than 100 ug/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously