Lipoprotein, Low Density, Human, Cu oxidized (LDL)

Lyophilized powder may be stored at -20 °C. Stable for 12 months at -20 °C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 °C. Reconstituted product is stable for 12 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recognizes fully oxidized modifications of human LDL including Cu2+-oxidized LDL, MDA-LDL, HOCL-LDL, but not to other oxidized proteins like MDA-HSA, HOCL-HSA, MDA-HDL, HOCL-HDL (below detection limit). The reaction of native LDL was weak, but clearly detectable (approx. 20%). Minimally oxidized LDL gave a strong binding signal (> 80%).