MDM2, phosphorylated (Ser185) (Murine Double Minute-2)

May be stored at 4 °C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 °C. Aliquots are stable for 12 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Recognizes mouse MDM 2 showing a band at ~102kD corresponding to phosphorylated MDM2. Cellular Localization: Nuclear and cytoplasmic. Expressed predominantly in the nucleoplasm. Interaction with ARF (P14) results in the localization of both proteins to the nucleus. The nucleolar localization signals in both ARF and MDM2 may be necessary to allow efficient nucleolar localization of both proteins.

[Mdm2; E3 ubiquitin-protein ligase Mdm2; E3 ubiquitin-protein ligase Mdm2; oncoprotein Mdm2; MDM2 variant FB28; MDM2 variant FB30; OTTHUMP00000183488; OTTHUMP00000183489; OTTHUMP00000183495; OTTHUMP00000183496; OTTHUMP00000240280; OTTHUMP00000240285; OTTHUMP00000240290; ubiquitin-protein ligase E3 Mdm2; double minute 2, human homolog of; p53-binding protein; Mdm2, transformed 3T3 cell double minute 2, p53 binding protein; Mdm2 p53 binding protein homolog (mouse); Double minute 2 protein; Hdm2; Oncoprotein Mdm2; p53-binding protein Mdm2]