Endophilin, pan, SH3 Domain

May be stored at 4 °C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 °C. Aliquots are stable for at least 12 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Reacts with mouse endophilin 1, 2 and 3 proteins. Based on 100% sequence homology, it is expected to cross-react with human and rat samples. On Western blots of mouse brain homogenates, two bands at ~40kD (endophilin 1, 2) and one band at ~45kD (endophilin 3) are identified. A faint band at ~52-55kD is also observed, which may represent an isomer. Reactivity has been confirmed with mouse brain lysates.