Anti - ATPase, Ca, Autoinhibitory Domain (c28r2)

May be stored at 4 °C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 °C. Aliquots are stable for at least 12 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

The 28 AA human C28R2 sequence is 100% conserved in human Ca+-ATPase isoforms 1, 2, 3b, 96% in 1b, 92% in 4 and 85% in 3a/3b. It is also 100% conserved in mouse and rat isoform 2. A high degree of conservation (77-100%) is also seen in Ca+-ATPase isoforms from many other species. Antibody crossreactivity of C28R2 or its exchange inhibitory properties in various species has not been studied. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity