Neprilysin-Like Protease, alpha (NEPL-a)

May be stored at 4 °C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 °C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 

The antigenic/control peptide is 100% conserved in mouse NEPL-a, b and g. No significant sequence homology of is seen with other proteins. Antibody reactivity in various species is not known. Control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking (use 5-10 ug per 1 ul of antiserum or 1 ug of aff pure IgG) to confirm antibody specificity (see below)