Anti - Neurite Outgrowth Pnhibitor Protein A,B,C (Nogo-A, B, C)

May be stored at 4 °C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 °C. Aliquots are stable for at least 12 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

The human peptide sequence is conserved in all 3 isoforms (Nogo A-C). It is 92% identical in rat Nogo or rat foocen-m1/2 protein. Within the 25-aa region identified for the inhibition of neurite outgrowth (1). Antibody crossreactivity of NogoA12 in various species is not established. Control peptides, because of their low MW (<3kD), are not suitable for Western. It should be used for ELISA or antibody blocking to confirm antibody specificity