Anti - Natural Resistance Associated Macrophage Protein (Nramp1)

May be stored at 4 °C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 °C. Aliquots are stable for at least 12 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

The 21 AA mouse control peptide sequence is 59% conserved in red deer, bison, sheep, bovine, 57% in human NRAMP1. No significant sequence homology exist with NRAMP2. Control peptide, because of its low MW (<3kD), is not suitable for Western. It is recommed for ELISA, dot blot, or for antibody blocking experiments (use 5-10 ug of control peptide per 1 ug of aff pure IgG or 1 ul of antiserum) to demonstrate antibody specificity

[natural resistance associated macrophage protein; Natural resistance-associated macrophage protein 1; natural resistance-associated macrophage protein 1; NRAMP 1; OTTMUSP00000017968; host resistance locus Bcg/Ity/Lsh; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 1]