Anti - Neprilysin-Like Protease, gamma (NEPL-g)

May be stored at 4 °C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 °C. Aliquots are stable for at least 12 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

The antigenic/control peptide is conserved in mouse (100%) NEPL-g. No significant sequence homology of is seen with other proteins. Antibody reactivity in various species is not known. The control peptide, because of its low MW (<3kD), is not suitable for Western. It should be used for ELISA or antibody blocking experiments (use 5-10 ug control peptide per 1 ug of aff pure IgG or 1 ul antiserum) to confirm antibody specificity (see below).