WASP, N-, non-phosphorylated (Ser-484/Ser-485) (WAS, THC, IMD2, WASP, Eczema-thrombocytopenia)

May be stored at 4 °C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 °C. Aliquots are stable for at least 12 months at -20 °C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Cross-absorbed to phospho-N-WASP (Ser-384/Ser-385) then affinity purified using nonphospho-N-WASP (Ser-384/Ser-385) peptide. The antibody detects a 65 kDa protein on SDS-PAGE immunoblots of rat brain and SKN-SH cell lysates that are treated with alkaline phosphatase. Only low levels of unphosphorylated WASP or N-WASP are detected in untreated rat brain, Jurkat, and A431 cells.